High-throughput gene platform for validating resistance against fusarium in corn

Fusarium species and other fungal pathogens are a persistent constraint to corn production in the Upper Midwest, including Minnesota. Fusarium infections cause stalk and ear rots, and accumulation of poisonous secondary metabolites (mycotoxins) that cause human and livestock health disorders via contaminated corn-based food and feeds. Fusarium species are a problem in the Northern states (which are cooler) and Aspergillus species are more of a problem in the Southern states (which are warmer). Unfortunately, climate change is making it possible for Aspergillus species to expand their range to include the US Corn Belt states thereby creating the need for solutions to both pathogens.
Genetically encoded resistance has the ability to both preserve corn yields and reduce mycotoxin levels in grain, increasing farmers’ profits while supporting more sustainable farming practices. These benefits will help ensure that Minnesota corn producers remain competitive in the global marketplace.
The Corn Mycotoxin Mitigation Initiative, led by the non-profit biotech company, 2Blades, is developing corn varieties with resistance to fungi that produce mycotoxins. In this program, 2Blades will use its high-throughput resistance gene discovery platforms, NLRseek™ and PRRseek™, in its laboratory at the University of Minnesota to identify novel sources of genetic resistance to mycotoxigenic fungi. The Cereals NLRseek™ platform is a collection of 985 unique, prioritized receptor proteins from eighteen grass families, introduced individually into wheat lines. Initial screening of this Wheat Array using Fusarium gramine arum in 2023 produced first candidates; we now aim to test an expanded set for resistance against strains of Fusarium species. The expanded tests will identify lead genes for introduction into corn. The program will be implemented with a product-oriented strategy, leveraging 2Blades’ network of private-sector seed and international agricultural organizations as delivery partners. The overarching goal of this program is to engineer Fusarium resistant corn varieties.